A novel reusable PAni-PVA-Amylase film: activity and analysis.

نویسندگان

  • Susmita Singh
  • Jyoti Prasad Saikia
  • Alak Kumar Buragohain
چکیده

Alpha-amylase was immobilized onto polyaniline-polyvinyl alcohol (PAni-PVA) film by cross linking with glutaraldehyde. The activity yield and immobilization efficiency was calculated as 13.58% and 30.60% respectively. The PAni-PVA-Amylase films were characterized by Fourier Transform Infrared Spectroscopy (FTIR) and amylase activity assay. The immobilized α-amylase had its optimum activity at pH 7.0 and 25°C. The immobilization of α-amylase onto the PAni-PVA films protected the enzyme from Fe(2+) inhibition. The PAni-PVA-Amylase films could be used effectively for 30 cycles with 60% retention of the activity.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Synthesis and Characterization of Peroxide Sensor Based on Pva-pani Nanocomposite

Peroxidase has been immobilized onto the PVA-PANI nanocomposite film deposited onto gold plate via physical adsorption for peroxide detection. PVA-PANI nanocomposite was characterized by scanning electron micrograph (SEM) before and after immobilization. The SEM picture clearly shows the immobilization of peroxidase onto the PVA-PANI. The cyclic voltametric study is also carried out for the as ...

متن کامل

Role of Nano-Sized TiO2 on Mechanical and Thermal Behavior of Starch/Poly (vinyl alcohol) Blend Films

A novel Starch/Poly (vinyl alcohol)/nano-Titanium dioxide (ST/PVA/nano-TiO2) biodegradable nanocomposite film was prepared by homogeneously dispersed TiO2 nanoparticles in different ratios of starch/PVA-based materials, via a solution casting method. Glycerol was used as plasticizer. The mechanical and thermal properties of films were studied using tensile and perforation strength tests and the...

متن کامل

The effect of temperature and water absorption on enzymatic degradation of starch / polyvinyl alcohol blend film by α-Amylase

Thermoplastic starch (TPS) materials present several advantages to the plastic industry andwhen blended with other materials they can exhibit improved mechanical and moisturesensitivity properties compared to pure TPS materials. Further investigations on TPS: PVAblends are of particular interest due to their excellent compatibility and improved properties suchas tensile strength, elongation, to...

متن کامل

Influence of a Novel Magnetic Recoverable Support on Kinetic, Stability and Activity of Beta-amylase Enzyme

In this paper, covalent immobilization of beta amylase enzyme on the surface of modified magnetic nano particles (ZnFe2O4@SiO2-NH2) is reported. For doing so, at first, the magnetic nanoparticles of ZnFe2O4 were synthesized by chemical co-precipitation method and then tetraethyl orthosilicate (TEOS) and 3-aminopropyltriethoxy sil...

متن کامل

Semi-IPN Films and Electrospun Nanofibers Based On Chitosan/PVA as an Antibacterial Wound Dressing

The antimicrobial activity of a wound dressing is a key factor for preventing and treating wound infection. The current study evaluated the physiochemical properties and antimicrobial activities of semi-IPNs (interpenetrating polymer networks) based on chitosan/polyvinyl alcohol (PVA) films and nanofibers as candidates for wound dressings and investigated the effects of morphologies (nanofibrou...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Colloids and surfaces. B, Biointerfaces

دوره 106  شماره 

صفحات  -

تاریخ انتشار 2013